Français
English
Products
DI Mycobasterium Tuberculosis complex High Performances (DIMTB-HPE)
- qPCR Duplex High sensitivity detection of Mycobacterium tuberculosis complex DNA.
- High sensitivity 2 targeted sequences on IS6110 gene. LoD: 9.38 genome copies/mL of sputum.
- Various sample types DNA extracted from sputum, sputum swab, tongue
swab, broncho-alveolar lavage samples. - Adaptable Easy to use and fast (1 hour post-extraction), transportable at room temperature (freeze dried), adaptable to different thermocyclers (MIC, CFX96, QS5) and high throughput.
- Robust quality control Amplification control. Exogenous internal control. UNG system to prevent carryover contamination.
- Simple pre-analytical steps Optimised DNA extraction with minimal steps and limited tube opening.
Learn more
The DI Mycobacterium Tuberculosis Complex High Performance (DIMTB-HPE) kit is an in vitro diagnostic device intended for the qualitative detection of DNA from bacteria responsible for respiratory infections in human patients presenting clinical signs compatible with tuberculosis, without restriction of age or associated pathology.
This device is based on real-time PCR technology. It allows the detection, in a single well, of bacteria belonging to the Mycobacterium tuberculosis complex (MTB: M. tuberculosis, M. bovis, M. africanum, M. microti, M. canettii, M. caprae, and M. pinnipedii) without species distinction, as well as a non-target exogenous control, co-extracted with the samples. This control is used to evaluate extraction efficiency and to detect the presence of potential PCR inhibitors.
The device is designed for use on sputum, bronchoalveolar lavage, nasopharyngeal, or tongue swabs collected by a qualified healthcare professional. Prior to PCR amplification, nucleic acids (NA) must be extracted using validated methods.
Features
| Samples |
Sputum, tongue swab, sputum swab, bronchoalveolar lavage |
| Targets |
3 different targets on the IS6110 gene and Mycobacterium smegmatis as NTPC |
| Controls | 3 different targets on the IS6110 gene and Mycobacterium smegmatis as NTPC |
| Duration | ~ 1 hour |
| Equipment | Nucleic acid extraction kit, thermocycler |
| Storage temperature | From -16°C to -26°C |
| Transport temperature | Room temperature |
| Reference | DIMTB-HPE-50 |
